Supplementary Table 5b. Expression profiles of KRAS

Supplementary Table 5c. Expression profiles of p53

Supplementary Table 5a. Expression profiles of EGFR

Supplementary Table 1. 50 probe sets reporting the greatest increase in BSM vs. SCC  samples

Supplementary Table 2. 50 probe sets reporting the greatest increase in SCC vs. BSM samples

Suppinfo1: Effects of coculture of breast MCF10A on the genomic profile of fibroblasts. Fifty-eight genes were significantly differentially expressed in cocultivated CAFs when compared with CAF monocultures but  this was filtered to 34 after applying a 2 fold cut-off

Suppinfo2: The influence of MDA-MB231 cells on gene expression of  carcinoma-associated fibroblasts CAFs.  Following the 2-fold cut-off, 4 were over expressed and 19 were under-expressed. Ontology analyses identified genes associated to cell cycle including TACC1 and CDKN1B genes, or to protein transport (TIMM17A) or classified under the category of integral to membrane (BAMBI).

Suppinfo3:  In MCF10A cocultured with Carcinoma associated fibroblasts (CAF) after a 2-fold cut-off, we found more genes upregulated (n = 77) and less genes downregulated (n = 21).

Suppinfo4: MCF10A and breast benign-disease adjacent tissues (NAF) coculture 48 upregulated and 33 downregulated genes

Suppinfo5: There were 35 upregulated and 55 downregulated genes in MDA-MB231 cocultured with CAF

Suppinfo6: Effects of coculture of normal fibroblasts on the expression profile of MDA-MB231. MDA-MB231/NAF presented 115 genes whose expression levels were altered. After applying the 2-fold change cut-off, 29 genes were upregulated and 50 were downregulated

Suppinfo7:  Effects of coculture of breast MCF10A on the genomic profile of fibroblasts. gene expression profile of  fibroblasts isolated from breast benign-disease adjacent tissues (NAF) grown alone or in coculture with MCF10A, just 7 genes were identified showing a 2-fold change

Suppinfo8: Effects of coculture of MDA-MB231 with fibroblasts isolated from breast benign-disease adjacent tissues (NAF).  The influence of MDA-MB231 cells on NAF resulted in downregulation of 8 of 9 differentially expressed genes (89%). Genes with decreased expression as determined by gene ontology analysis were TSPAN5 and PLOD whereas TACC2 was upregulated.